Saturday, 24 Oct 2020
Page: 87
Volume 28, Issue 10(209) 2018

Effect of Zn2+ and nanocurcumin on the development of antibiotic resistance in Staphylococcus aureus

Author: Nguyen Ngoc Thuy Giang, Le Quy Khang and Nguyen Thi Thu Hoai
Combating the threat of antibiotic resistance has been the major focus of medical research recently due to the widespread of multidrug resistance and scarcity of newly introduced antimicrobials. This study aimed to investigate the effectiveness of Zn2+ and nanocurcumin in delaying antibiotic resistance development in Staphylococcus aureus. To do this, S. aureus ATCC®29213 was exposed to sub-inhibitory concentrations of flouroquinolones either ciproflocaxin, levofloxacin or ofloxacin in the presence of either Zn2+ or nanocurcumin. Minimal inhibitory concentrations (MIC) of each antibiotics were measured each day in 14 days of antibiotic exposure and expression of recA gene, an important gene in antimicrobial resistance development, was monitored using reverse transcription real-time quantitative PCR (RT-qPCR). Results showed that the addition of Zn2+ (400 μM) into fluoroquinolone antibiotics was helpful in reducing the rate and magnitude of antibiotic resistance development. Nanocurcumin (8 μg/mL), however, showed no significant effect on the resistance development process. Furthermore, in the presence of Zn2+ but not nanocurcumin, the expression of recA was repressed by up to 15-fold compared to the control. In summary, our study strongly supports the combination therapy, Zn2+ and antibiotics, for effective treatment of S. aureus infections
Antibiotic resistance; nanocurcumin; zinc; recA; Staphylococcus aureus
.pdf 10. Nguyen Thi Thu Hoai.pdf
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