Saturday, 24 Oct 2020
Page: 74
Volume XXVI, Issue 12(185) 2016

The comparison of sporicidal activity among different disinfectants used at hospitals in Hanoi, Vietnam against Clostridium difficile spores

Author: Vu Thi Thu Huong, Tang Thi Nga, Phung Thi Thu Hang, Kieu Chi Thanh and Dang Duc Anh
Clostridium difficile (C. difficile) has become the leading cause of nosocomial diarrheas worldwide. In Vietnam, there is no data for evaluating sporicidal activity against C. difficile spores of disinfectants. Twelve disinfectants were selected based on guidelines of Vietnam Ministry of Health and the absence of validated data. To evaluate the sporicidal effect of disinfectants, a suspension test method was applied using pure spores of C. difficile. The results showed that of the 12 disinfectants, only 4 disinfectants met the requirement of reduction in viability of C. difficile spores (>=105-fold) for contact time of 5 min, under both clean (0.3% albumin) and dirty (3% albumin) conditions. These disinfectants were sodium hypochloride 0.5%, Chloramin B 0.5%, Ortho-phthalaldehyde 0.55%, and Peracetic acid 0.15%. In contrast, 2 disinfectants (Chlohexidin gluconate) and 5 disinfectants (quaternary ammonium compounds) showed little or no measurable sporicidal activity after 5 and 60 min. No difference was found regarding susceptibility to different disinfectants among the pure spores of nontoxigenic C. difficle ATCC 700057, CDF 18 (A+B+CDT-PCR ribotype cc835) and CDF1150 (A-B+CDT-PCR ribotype 017) strains. Among 21 hospitals in Hanoi, 14 hospitals are currently using 4 appropriate disinfectants against C. difficile spores for treating both surfaces and endoscopes/equipments in the environments. In conclusion, Javen and Chloramin B were the most effective agents for eliminating C. difficle spores in environmental surfaces, while Peracetic and Sanidex OPA solution were the most appropriate disinfectants for instruments and other hospital facilities. These four disinfectants could be chosen for routine disinfection in medical facilities in Vietnam.
Clostridium difficile, spore, disinfectant, sporicidal test
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Happy New Year 2019! Year of the Pig
The Vietnam Journal of Preventive Medicine wish all readers with healthy, happiness and prosperity in the year of the Pig. Happy New Year 2019
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