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Page: 24
Volume XXVI, Issue 12(185) 2016
Comparison of laboratory methods for measles diagnosis in Northern Vietnam, 2014
Author: Do Phuong Loan, Nguyen Minh Hang, Trieu Thi Thanh Van, Nguyen Thi Mai Duyen, Komase Katsuhiro and Nguyen Tran Hien
Summary:
Measles virus is one of the major causes of rash and fever in children in developing countries. Although measles vaccines and measles-containing vaccines were well-developed and widely used in children, measles virus still caused outbreaks worldwide even where the vaccine coverage are high. In 2014, there were big outbreaks occurring in many countries, including Vietnam. Laboratory results of measles cases played an important role in new outbreak cluster confirmation and monitoring. In this study, we compared different laboratory methods for measles infection to provide information for measles diagnostic strategy. Out of 4,672 suspected cases, laboratory-confirmed measles individuals were 63.0% in which 89% were confirmed by IgM ELISA methods. The detection rate of virus isolation and RT-PCR on respiratory tract samples was 7.3% and 71.4%, respectively. On serum samples, the detection rate of ELISA and nested RT-PCR (nRT-PCR) was 62.1% and 19.6%, respectively. Using ELISA on serum samples collected in acute phase of measles infection could miss about 18.0% of measles cases. The application of nRT-PCR on acute serum will improve the measles virus genotype information and supplement results for serological methods.
Keywords:
Measles, ELISA, nested RT-PCR, RT-PCR
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E161224.pdf
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Happy New Year 2019! Year of the Pig
The Vietnam Journal of Preventive Medicine wish all readers with healthy, happiness and prosperity in the year of the Pig. Happy New Year 2019
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