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Page: 29
Volume 1E, Issue 1(1) 2014

Comparison of three selective media for the recovery of Clostridium difficile from stool specimens

Author: Vu Thi Thu Huong, Tang Thi Nga, Le Thi Trang, Nguyen Binh Minh, Nguyen Bich Van, Nguyen Van Kinh and Dang Duc Anh
Summary:
There is a need for effective, economical and readily available selective media for the culture of Clostridium difficile.This study aimed to evaluate the performance of cycloserin-cefoxitin-mannitol agar (in-house CCMA) and cycloserin-cefoxitin-mannitol broth (in-house CCMB), compared to cycloserin-cefoxitin-fructose agar (CCFA, commercial medium for the recovery of C. difficile from stool specimens.The average size of C. difficile colonies on CCMA were bigger compared to those grown on CCFA at 48h of incubation. Bacterial numbers of C. difficile on CCMA was more than on CCFA for 2 stool specimens at 48h (55 colonies on CCMA vs. 9 colonies on CCFA). Of 58 stool samples tested, CCMA and CCMB enhanced C. difficile growth and improved recovery 9 times higher than that on CCFA (18 isolates on CCMA/CCMB vs. 2 isolates on CCFA. Because of the ease of use and lower cost, CCMA was preferable medium to CCMB. Our results suggest that our in house CCMA is sensitive and reduced-cost medium for the recovery of C. difficile from stool specimens.
Keywords:
Clostridium difficile; cycloserin-cefoxitin-fructose agar; cycloserin-cefoxitin-mannitol broth; cycloserin-cefoxitin-mannitol agar
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Happy New Year 2019! Year of the Pig
The Vietnam Journal of Preventive Medicine wish all readers with healthy, happiness and prosperity in the year of the Pig. Happy New Year 2019
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